Journal: STAR Protocols

Article Title: Ligature-induced periodontitis mouse model protocol for studying Saccharibacteria

doi: 10.1016/j.xpro.2022.101167

Figure Lengend Snippet:

Article Snippet: Defibrinated sheep blood , Northeast Laboratory Services , SB100.

Techniques: Purification, Polymer, Plasmid Preparation, DNA Purification, Bacteria, Recombinant, Saline, Microscopy, Transferring, Adhesive, Infection, Filtration, Spectrophotometry, Software

Journal: Nature protocols

Article Title: Near-infrared light–controlled systems for gene transcription regulation, protein targeting and spectral multiplexing

doi: 10.1038/nprot.2018.022

Figure Lengend Snippet: Plasmids used in the protocol.

Article Snippet: Transcription from the minimal promoter is activated by the p65 transactivation domain. a adapted from ref. 5 , Nature Publishing Group. table ft1 table-wrap mode="anchored" t5 Table 1 | caption a7 Application Plasmid Description Addgene no. Reference Transcription activation (two-color), Steps 3, 7A pQP-T2A BphP1–VP16–T2A–NLS–GAL4–QPAS1 102583 5 pGAVPO GAL4–VVD–p65 Not commercially available a 3 pFR-Luc UAS-controlled reporter (luciferase) Not available from Addgene b 5 Tridirectional protein targeting, Steps 3, 7B pQP-iRIS BphP1–mVenus–CAAX–IRES2-NES–mCherry–QPAS1–AsLOV2NLS 102584 5 Stable cell line generation, Box 1 pT2/SVNeo-103 RpBphP1–mCherry–TetR 79841 4 pCMV(CAT)T7-SB100 Sleeping Beauty 100× transposase 34879 45 Kinetics of light-induced transcription activation, Box 2 pCMV-104 NLS–PpsR2–VP16 79842 4 pTRE-Tight-SEAP TRE-controlled reporter (alkaline phosphatase) Not commercially available a 4 Light activation of gene expression in mice, Box 3 pCMV-104 NLS–PpsR2–VP16 79842 4 pTRE-Tight-Rluc8 TRE-controlled reporter (luciferase) Not commercially available a 4 Open in a separate window a Plasmids and plasmid sequences should be requested from the corresponding authors of the respective original publications. b Available from Agilent Technologies, cat. no. 219050.

Techniques: Plasmid Preparation, Activation Assay, Luciferase, Stable Transfection, Expressing